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αgfp hrp antibodies  (Miltenyi Biotec)
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Miltenyi Biotec αgfp hrp antibodies
The pool of Cf-4 and <t>Cf-9</t> <t>proteins</t> interacting with SOBIR1 is larger than that of Cf-2 and Cf-5. (a ) Cf-4, Cf-9, Cf-2, and Cf-5, all tagged with eGFP, were co-expressed with Sl SOBIR1-Myc in leaves of N. benthamiana . After 2 dpi, total protein was extracted and incubated with <t>GFP</t> beads for immunoprecipitation. The immunoprecipitated proteins were subjected to immunoblotting with αGFP antibodies to visualize the eGFP-tagged Cf proteins and with αMyc antibodies to detect the Myc-tagged SOBIR1 protein. Rubisco bands indicate the total protein content of the input samples. b and c) Cf-4-eGFP, Cf-5-eGFP, and Cf-2-eGFP were transiently expressed in leaves of N. benthamiana , and at 2 dpi, total proteins were extracted and subjected to immunoprecipitation using GFP beads. Peptides were obtained from the immunoprecipitated proteins by a tryptic digest and were analyzed by LFQ nano-MS/MS. b) Comparison of the co-immunopurified proteins between Cf-4 and Cf-5, and c) between Cf-4 and Cf-2, by volcano plots. Significantly enriched proteins are indicated by the black dots. The light gray dots represent proteins that are not significantly enriched. Note that with Cf-4-eGFP, more SOBIR1 protein is purified than with Cf-5-eGFP or Cf-2-eGFP.
αgfp Hrp Antibodies, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+gfp/pmc13425104-245-7-9?v=Miltenyi+Biotec
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αgfp hrp antibodies - by Bioz Stars, 2026-08
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primary antibody goat anti gfp  (Rockland Immunochemicals)
96
Rockland Immunochemicals primary antibody goat anti gfp
The pool of Cf-4 and <t>Cf-9</t> <t>proteins</t> interacting with SOBIR1 is larger than that of Cf-2 and Cf-5. (a ) Cf-4, Cf-9, Cf-2, and Cf-5, all tagged with eGFP, were co-expressed with Sl SOBIR1-Myc in leaves of N. benthamiana . After 2 dpi, total protein was extracted and incubated with <t>GFP</t> beads for immunoprecipitation. The immunoprecipitated proteins were subjected to immunoblotting with αGFP antibodies to visualize the eGFP-tagged Cf proteins and with αMyc antibodies to detect the Myc-tagged SOBIR1 protein. Rubisco bands indicate the total protein content of the input samples. b and c) Cf-4-eGFP, Cf-5-eGFP, and Cf-2-eGFP were transiently expressed in leaves of N. benthamiana , and at 2 dpi, total proteins were extracted and subjected to immunoprecipitation using GFP beads. Peptides were obtained from the immunoprecipitated proteins by a tryptic digest and were analyzed by LFQ nano-MS/MS. b) Comparison of the co-immunopurified proteins between Cf-4 and Cf-5, and c) between Cf-4 and Cf-2, by volcano plots. Significantly enriched proteins are indicated by the black dots. The light gray dots represent proteins that are not significantly enriched. Note that with Cf-4-eGFP, more SOBIR1 protein is purified than with Cf-5-eGFP or Cf-2-eGFP.
Primary Antibody Goat Anti Gfp, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+gfp/pmc12848578-176-14-18?v=Rockland+Immunochemicals
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primary antibody goat anti gfp - by Bioz Stars, 2026-08
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gfp hrp  (Miltenyi Biotec)
96
Miltenyi Biotec gfp hrp
The pool of Cf-4 and <t>Cf-9</t> <t>proteins</t> interacting with SOBIR1 is larger than that of Cf-2 and Cf-5. (a ) Cf-4, Cf-9, Cf-2, and Cf-5, all tagged with eGFP, were co-expressed with Sl SOBIR1-Myc in leaves of N. benthamiana . After 2 dpi, total protein was extracted and incubated with <t>GFP</t> beads for immunoprecipitation. The immunoprecipitated proteins were subjected to immunoblotting with αGFP antibodies to visualize the eGFP-tagged Cf proteins and with αMyc antibodies to detect the Myc-tagged SOBIR1 protein. Rubisco bands indicate the total protein content of the input samples. b and c) Cf-4-eGFP, Cf-5-eGFP, and Cf-2-eGFP were transiently expressed in leaves of N. benthamiana , and at 2 dpi, total proteins were extracted and subjected to immunoprecipitation using GFP beads. Peptides were obtained from the immunoprecipitated proteins by a tryptic digest and were analyzed by LFQ nano-MS/MS. b) Comparison of the co-immunopurified proteins between Cf-4 and Cf-5, and c) between Cf-4 and Cf-2, by volcano plots. Significantly enriched proteins are indicated by the black dots. The light gray dots represent proteins that are not significantly enriched. Note that with Cf-4-eGFP, more SOBIR1 protein is purified than with Cf-5-eGFP or Cf-2-eGFP.
Gfp Hrp, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gfp hrp - by Bioz Stars, 2026-08
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anti gfp magnetic beads  (Abbkine Inc)
86
Abbkine Inc anti gfp magnetic beads
The pool of Cf-4 and <t>Cf-9</t> <t>proteins</t> interacting with SOBIR1 is larger than that of Cf-2 and Cf-5. (a ) Cf-4, Cf-9, Cf-2, and Cf-5, all tagged with eGFP, were co-expressed with Sl SOBIR1-Myc in leaves of N. benthamiana . After 2 dpi, total protein was extracted and incubated with <t>GFP</t> beads for immunoprecipitation. The immunoprecipitated proteins were subjected to immunoblotting with αGFP antibodies to visualize the eGFP-tagged Cf proteins and with αMyc antibodies to detect the Myc-tagged SOBIR1 protein. Rubisco bands indicate the total protein content of the input samples. b and c) Cf-4-eGFP, Cf-5-eGFP, and Cf-2-eGFP were transiently expressed in leaves of N. benthamiana , and at 2 dpi, total proteins were extracted and subjected to immunoprecipitation using GFP beads. Peptides were obtained from the immunoprecipitated proteins by a tryptic digest and were analyzed by LFQ nano-MS/MS. b) Comparison of the co-immunopurified proteins between Cf-4 and Cf-5, and c) between Cf-4 and Cf-2, by volcano plots. Significantly enriched proteins are indicated by the black dots. The light gray dots represent proteins that are not significantly enriched. Note that with Cf-4-eGFP, more SOBIR1 protein is purified than with Cf-5-eGFP or Cf-2-eGFP.
Anti Gfp Magnetic Beads, supplied by Abbkine Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti gfp magnetic beads - by Bioz Stars, 2026-08
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anti gfp magnetic ip kit  (Abbkine Inc)
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Abbkine Inc anti gfp magnetic ip kit
The pool of Cf-4 and <t>Cf-9</t> <t>proteins</t> interacting with SOBIR1 is larger than that of Cf-2 and Cf-5. (a ) Cf-4, Cf-9, Cf-2, and Cf-5, all tagged with eGFP, were co-expressed with Sl SOBIR1-Myc in leaves of N. benthamiana . After 2 dpi, total protein was extracted and incubated with <t>GFP</t> beads for immunoprecipitation. The immunoprecipitated proteins were subjected to immunoblotting with αGFP antibodies to visualize the eGFP-tagged Cf proteins and with αMyc antibodies to detect the Myc-tagged SOBIR1 protein. Rubisco bands indicate the total protein content of the input samples. b and c) Cf-4-eGFP, Cf-5-eGFP, and Cf-2-eGFP were transiently expressed in leaves of N. benthamiana , and at 2 dpi, total proteins were extracted and subjected to immunoprecipitation using GFP beads. Peptides were obtained from the immunoprecipitated proteins by a tryptic digest and were analyzed by LFQ nano-MS/MS. b) Comparison of the co-immunopurified proteins between Cf-4 and Cf-5, and c) between Cf-4 and Cf-2, by volcano plots. Significantly enriched proteins are indicated by the black dots. The light gray dots represent proteins that are not significantly enriched. Note that with Cf-4-eGFP, more SOBIR1 protein is purified than with Cf-5-eGFP or Cf-2-eGFP.
Anti Gfp Magnetic Ip Kit, supplied by Abbkine Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+gfp/pmc13254979-52-0-5?v=Abbkine+Inc
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anti gfp magnetic ip kit - by Bioz Stars, 2026-08
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resource source identifier antibodies anti gfp hrp antibody miltenyi biotec  (Miltenyi Biotec)
96
Miltenyi Biotec resource source identifier antibodies anti gfp hrp antibody miltenyi biotec
The pool of Cf-4 and <t>Cf-9</t> <t>proteins</t> interacting with SOBIR1 is larger than that of Cf-2 and Cf-5. (a ) Cf-4, Cf-9, Cf-2, and Cf-5, all tagged with eGFP, were co-expressed with Sl SOBIR1-Myc in leaves of N. benthamiana . After 2 dpi, total protein was extracted and incubated with <t>GFP</t> beads for immunoprecipitation. The immunoprecipitated proteins were subjected to immunoblotting with αGFP antibodies to visualize the eGFP-tagged Cf proteins and with αMyc antibodies to detect the Myc-tagged SOBIR1 protein. Rubisco bands indicate the total protein content of the input samples. b and c) Cf-4-eGFP, Cf-5-eGFP, and Cf-2-eGFP were transiently expressed in leaves of N. benthamiana , and at 2 dpi, total proteins were extracted and subjected to immunoprecipitation using GFP beads. Peptides were obtained from the immunoprecipitated proteins by a tryptic digest and were analyzed by LFQ nano-MS/MS. b) Comparison of the co-immunopurified proteins between Cf-4 and Cf-5, and c) between Cf-4 and Cf-2, by volcano plots. Significantly enriched proteins are indicated by the black dots. The light gray dots represent proteins that are not significantly enriched. Note that with Cf-4-eGFP, more SOBIR1 protein is purified than with Cf-5-eGFP or Cf-2-eGFP.
Resource Source Identifier Antibodies Anti Gfp Hrp Antibody Miltenyi Biotec, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+gfp/pm42320466-157-2-8?v=Miltenyi+Biotec
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resource source identifier antibodies anti gfp hrp antibody miltenyi biotec - by Bioz Stars, 2026-08
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anti gfp magbeads  (Yeasen Biotechnology)
86
Yeasen Biotechnology anti gfp magbeads
The pool of Cf-4 and <t>Cf-9</t> <t>proteins</t> interacting with SOBIR1 is larger than that of Cf-2 and Cf-5. (a ) Cf-4, Cf-9, Cf-2, and Cf-5, all tagged with eGFP, were co-expressed with Sl SOBIR1-Myc in leaves of N. benthamiana . After 2 dpi, total protein was extracted and incubated with <t>GFP</t> beads for immunoprecipitation. The immunoprecipitated proteins were subjected to immunoblotting with αGFP antibodies to visualize the eGFP-tagged Cf proteins and with αMyc antibodies to detect the Myc-tagged SOBIR1 protein. Rubisco bands indicate the total protein content of the input samples. b and c) Cf-4-eGFP, Cf-5-eGFP, and Cf-2-eGFP were transiently expressed in leaves of N. benthamiana , and at 2 dpi, total proteins were extracted and subjected to immunoprecipitation using GFP beads. Peptides were obtained from the immunoprecipitated proteins by a tryptic digest and were analyzed by LFQ nano-MS/MS. b) Comparison of the co-immunopurified proteins between Cf-4 and Cf-5, and c) between Cf-4 and Cf-2, by volcano plots. Significantly enriched proteins are indicated by the black dots. The light gray dots represent proteins that are not significantly enriched. Note that with Cf-4-eGFP, more SOBIR1 protein is purified than with Cf-5-eGFP or Cf-2-eGFP.
Anti Gfp Magbeads, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti gfp magbeads - by Bioz Stars, 2026-08
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anti gfphrp immunoblotting  (Miltenyi Biotec)
96
Miltenyi Biotec anti gfphrp immunoblotting
The pool of Cf-4 and <t>Cf-9</t> <t>proteins</t> interacting with SOBIR1 is larger than that of Cf-2 and Cf-5. (a ) Cf-4, Cf-9, Cf-2, and Cf-5, all tagged with eGFP, were co-expressed with Sl SOBIR1-Myc in leaves of N. benthamiana . After 2 dpi, total protein was extracted and incubated with <t>GFP</t> beads for immunoprecipitation. The immunoprecipitated proteins were subjected to immunoblotting with αGFP antibodies to visualize the eGFP-tagged Cf proteins and with αMyc antibodies to detect the Myc-tagged SOBIR1 protein. Rubisco bands indicate the total protein content of the input samples. b and c) Cf-4-eGFP, Cf-5-eGFP, and Cf-2-eGFP were transiently expressed in leaves of N. benthamiana , and at 2 dpi, total proteins were extracted and subjected to immunoprecipitation using GFP beads. Peptides were obtained from the immunoprecipitated proteins by a tryptic digest and were analyzed by LFQ nano-MS/MS. b) Comparison of the co-immunopurified proteins between Cf-4 and Cf-5, and c) between Cf-4 and Cf-2, by volcano plots. Significantly enriched proteins are indicated by the black dots. The light gray dots represent proteins that are not significantly enriched. Note that with Cf-4-eGFP, more SOBIR1 protein is purified than with Cf-5-eGFP or Cf-2-eGFP.
Anti Gfphrp Immunoblotting, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+gfp/pm42310955-323-22-25?v=Miltenyi+Biotec
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anti gfphrp immunoblotting - by Bioz Stars, 2026-08
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anti gfp m2 affinity beads  (Yeasen Biotechnology)
86
Yeasen Biotechnology anti gfp m2 affinity beads
The pool of Cf-4 and <t>Cf-9</t> <t>proteins</t> interacting with SOBIR1 is larger than that of Cf-2 and Cf-5. (a ) Cf-4, Cf-9, Cf-2, and Cf-5, all tagged with eGFP, were co-expressed with Sl SOBIR1-Myc in leaves of N. benthamiana . After 2 dpi, total protein was extracted and incubated with <t>GFP</t> beads for immunoprecipitation. The immunoprecipitated proteins were subjected to immunoblotting with αGFP antibodies to visualize the eGFP-tagged Cf proteins and with αMyc antibodies to detect the Myc-tagged SOBIR1 protein. Rubisco bands indicate the total protein content of the input samples. b and c) Cf-4-eGFP, Cf-5-eGFP, and Cf-2-eGFP were transiently expressed in leaves of N. benthamiana , and at 2 dpi, total proteins were extracted and subjected to immunoprecipitation using GFP beads. Peptides were obtained from the immunoprecipitated proteins by a tryptic digest and were analyzed by LFQ nano-MS/MS. b) Comparison of the co-immunopurified proteins between Cf-4 and Cf-5, and c) between Cf-4 and Cf-2, by volcano plots. Significantly enriched proteins are indicated by the black dots. The light gray dots represent proteins that are not significantly enriched. Note that with Cf-4-eGFP, more SOBIR1 protein is purified than with Cf-5-eGFP or Cf-2-eGFP.
Anti Gfp M2 Affinity Beads, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


The pool of Cf-4 and Cf-9 proteins interacting with SOBIR1 is larger than that of Cf-2 and Cf-5. (a ) Cf-4, Cf-9, Cf-2, and Cf-5, all tagged with eGFP, were co-expressed with Sl SOBIR1-Myc in leaves of N. benthamiana . After 2 dpi, total protein was extracted and incubated with GFP beads for immunoprecipitation. The immunoprecipitated proteins were subjected to immunoblotting with αGFP antibodies to visualize the eGFP-tagged Cf proteins and with αMyc antibodies to detect the Myc-tagged SOBIR1 protein. Rubisco bands indicate the total protein content of the input samples. b and c) Cf-4-eGFP, Cf-5-eGFP, and Cf-2-eGFP were transiently expressed in leaves of N. benthamiana , and at 2 dpi, total proteins were extracted and subjected to immunoprecipitation using GFP beads. Peptides were obtained from the immunoprecipitated proteins by a tryptic digest and were analyzed by LFQ nano-MS/MS. b) Comparison of the co-immunopurified proteins between Cf-4 and Cf-5, and c) between Cf-4 and Cf-2, by volcano plots. Significantly enriched proteins are indicated by the black dots. The light gray dots represent proteins that are not significantly enriched. Note that with Cf-4-eGFP, more SOBIR1 protein is purified than with Cf-5-eGFP or Cf-2-eGFP.

Journal: Plant Physiology

Article Title: The C-terminal tail of Cf resistance proteins determines the intensity of the effector-triggered hypersensitive response-related cell death

doi: 10.1093/plphys/kiag476

Figure Lengend Snippet: The pool of Cf-4 and Cf-9 proteins interacting with SOBIR1 is larger than that of Cf-2 and Cf-5. (a ) Cf-4, Cf-9, Cf-2, and Cf-5, all tagged with eGFP, were co-expressed with Sl SOBIR1-Myc in leaves of N. benthamiana . After 2 dpi, total protein was extracted and incubated with GFP beads for immunoprecipitation. The immunoprecipitated proteins were subjected to immunoblotting with αGFP antibodies to visualize the eGFP-tagged Cf proteins and with αMyc antibodies to detect the Myc-tagged SOBIR1 protein. Rubisco bands indicate the total protein content of the input samples. b and c) Cf-4-eGFP, Cf-5-eGFP, and Cf-2-eGFP were transiently expressed in leaves of N. benthamiana , and at 2 dpi, total proteins were extracted and subjected to immunoprecipitation using GFP beads. Peptides were obtained from the immunoprecipitated proteins by a tryptic digest and were analyzed by LFQ nano-MS/MS. b) Comparison of the co-immunopurified proteins between Cf-4 and Cf-5, and c) between Cf-4 and Cf-2, by volcano plots. Significantly enriched proteins are indicated by the black dots. The light gray dots represent proteins that are not significantly enriched. Note that with Cf-4-eGFP, more SOBIR1 protein is purified than with Cf-5-eGFP or Cf-2-eGFP.

Article Snippet: Proteins tagged with eGFP were detected using αGFP-HRP antibodies (Miltenyi Biotec, 130-091-833), while αMyc antibodies (cMyc9E10, sc-40, Santa Cruz) were used to detect Myc-tagged proteins, followed by incubation with αMouse-HRP (GE Healthcare) as a secondary antibody.

Techniques: Incubation, Immunoprecipitation, Western Blot, Tandem Mass Spectroscopy, Comparison, Purification

The C-terminal tail of Cf proteins plays a role in the affinity of the Cf protein for SOBIR1. a) eGFP-tagged Cf-4 and Cf-5 and the various chimeric Cf proteins were transiently co-expressed with Myc-fused Sl SOBIR1 in leaves of N. benthamiana (OD 600 = 0.5). At 2 dpi, total protein was extracted and subjected to immunoprecipitation (IP) using GFP-trap beads, followed by western blotting with αGFP (upper panels) and αMyc (bottom panels) antibodies. b) Relative quantification of the amounts of Sl SOBIR1 protein co-purifying with Cf-4 and Cf-5 and with the chimeric Cf proteins. The relative amount of Sl SOBIR1 co-purifying with the wild-type Cf proteins was set to 1. An ANOVA/Dunnett's multiple comparison was used to determine statistical significance. Error bars display the SD. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Journal: Plant Physiology

Article Title: The C-terminal tail of Cf resistance proteins determines the intensity of the effector-triggered hypersensitive response-related cell death

doi: 10.1093/plphys/kiag476

Figure Lengend Snippet: The C-terminal tail of Cf proteins plays a role in the affinity of the Cf protein for SOBIR1. a) eGFP-tagged Cf-4 and Cf-5 and the various chimeric Cf proteins were transiently co-expressed with Myc-fused Sl SOBIR1 in leaves of N. benthamiana (OD 600 = 0.5). At 2 dpi, total protein was extracted and subjected to immunoprecipitation (IP) using GFP-trap beads, followed by western blotting with αGFP (upper panels) and αMyc (bottom panels) antibodies. b) Relative quantification of the amounts of Sl SOBIR1 protein co-purifying with Cf-4 and Cf-5 and with the chimeric Cf proteins. The relative amount of Sl SOBIR1 co-purifying with the wild-type Cf proteins was set to 1. An ANOVA/Dunnett's multiple comparison was used to determine statistical significance. Error bars display the SD. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Article Snippet: Proteins tagged with eGFP were detected using αGFP-HRP antibodies (Miltenyi Biotec, 130-091-833), while αMyc antibodies (cMyc9E10, sc-40, Santa Cruz) were used to detect Myc-tagged proteins, followed by incubation with αMouse-HRP (GE Healthcare) as a secondary antibody.

Techniques: Immunoprecipitation, Western Blot, Quantitative Proteomics, Comparison